Since 1959 ~ -aminoethylphosphonic acid was discovered in the living organism, the biosynthesis and biological functions of aminophosphonic acids have been extensively studied.
The author designed and carried out this study for 14 weeks to find out "lie metabolic function of Ethylaminophosphonic acid (AEP) and it´s utilization in the living body. Sixty rats, thirty males and thirty females aged 405 days were divided into two parts, one for alanine supplemented as control group and the other for AEP as experimental group to compare metabolic pathway of ordinary amino acid with that of AEP.
Both alamine and AEP group were divided into two subgroups according to the level of supplements, 0. 1% and 0. 25% of the diet. The major components of the diet in this study were composed of 20,0 casein, 72% Sugar, 4% fat, 4% salt Mixture, and all kind of Uitamins in adequate amount. For comparision of biological values between experimental and control group in terms of body weight, uninary nitrogen, creatinine excretion and final orgam weight, there were no statistically significant difference in these respects. This meant AEP could be utilized in the body as much as alanine could.
Urinary phosphorus excretion was determined by developing the blue color to read on the Spectronic 20. Statistically insignificance in the urinary phosphorus excretion between experimental and control group was observed in spite of the supplementation of phosphorus of AEP for experimental group in the diet. The level of blood phosphorus was higher in experimental group than that in control group this result supported above result.
In the analysis of fat and nitrogen contents in the liver, AEP group showed slightly higher than control in both respects. But it was noteworthy 0.2% AEP group in both sex were higher than 0. 1% AEP in liver fat content.
Histological examinal of internal organs liiver, lung, spleen, heart, kindey, adrenal and sex organs showed no changes in all groups included in this study. The group supplemented higher level of diet. by alanine 0. 2o and AEP 0. 2% stayed on less body weight gain and lower liver weight. This result could be interpreted that amino acid imbalanced condition was arose in the body.
|